Plasmid

Part:BBa_I713007:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-25)


pEB T7X / Amp Kan


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3008
    Illegal SpeI site found at 258
    Illegal SpeI site found at 2952
    Illegal PstI site found at 2968
    Illegal PstI site found at 4158
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 258
    Illegal SpeI site found at 2952
    Illegal PstI site found at 2968
    Illegal PstI site found at 4158
    Illegal NotI site found at 2989
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3945
    Illegal BamHI site found at 252
    Illegal BamHI site found at 294
    Illegal XhoI site found at 2996
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3008
    Illegal SpeI site found at 258
    Illegal SpeI site found at 2952
    Illegal PstI site found at 2968
    Illegal PstI site found at 4158
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3008
    Illegal SpeI site found at 258
    Illegal SpeI site found at 2952
    Illegal PstI site found at 2968
    Illegal PstI site found at 4158
    Illegal NgoMIV site found at 3334
    Illegal NgoMIV site found at 4609
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5508
    Illegal SapI.rc site found at 4458
    Illegal SapI.rc site found at 4668


Design Notes

Clone the T7 RNAP gene without ATG.


Source

pEASY-B Cloning vector E. coli BL21(DE3)

References